Fatty acid composition of oils from green coffee beans from different agro-ecological zones of Uganda
Abstract
Coffee is an important agricultural commodity and beverage widely consumed throughout the
world. Two-thirds of Ugandans depend on coffee as an important source of income. The quality
of coffee determines the relative price and its end use. The lipid composition has been used to
characterize Arabica and Robusta coffee. Oil yield, fatty acid (FA) composition and oil
nutritional quality of green coffee beans; arabica coffee (Coffea arabica) from Bugisu,
Rwenzori, Kisoro and Maracha, and robusta coffee (Coffea canephora var. robusta) from
Iganga, Ibanda, Rukungiri, Mbarara, Ntungamo, Masaka, Kayunga and Luwero coffee regions of
Uganda were determined. Green coffee beans were obtained from Uganda Coffee Development
Authority (UCDA) and coffee dealers in Iganga, Luwero, Kisoro and Maracha Districts. Oil was
extracted in a Soxhlet apparatus using n-hexane. Fatty acids were determined as the FA methyl
esters (FAME) using flame ionization detection gas chromatography. Differences in oil yield
were analysed by ANOV A. The PUF A/SaF A, MUF A/SaF A, (PUF A +MUF A)/SaF A and
PUF A/MUF A ratios were used to evaluate the nutritional quality of the oil. Oil yield ranged
between 1. 75 and 15 .45%. Arabica coffee from Bugisu gave a significantly (p < 0.05) higher oil
yield. Robusta coffee from Ntungamo gave the lowest mean oil yield. Unsaturated fatty acids
(UFA) predominated over saturated fatty acids (SaFA). Linoleic and oleic acids were the main
unsaturated fatty acids accounting for 53% of the total. Palmitic acid was the major SaFA. Oil
yield and FA composition were dependent on agro-ecological coffee sample source and coffee
variety. Based on the obtained nutritional quality indices, green coffee oil can be used in foods
on blending with other oils. Nevertheless, there is need to investigate the existence of the transfatty
acids in green coffee oil.
KEY WORDS: Coffea arabica, Coffea canephora, fatty acid, green coffee beans, oil yield